Composite

Part:BBa_K1615088:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-16)


TVEL5 laccase fused to Linker-CBDcipA-linker driven by LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2265
    Illegal BglII site found at 2426
    Illegal BamHI site found at 2489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2071


Design Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.


Source

TVEL5 Laccase from Tramates versicolor. CBDcipA from Clostridium thermocellum. Lac expression cassette from iGEM10_Washington.

References